Comparative kinetic studies on the two interconvertible forms of Streptococcus faecalis inorganic pyrophosphatase
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چکیده
منابع مشابه
Effects of spermine and spermidine on the inorganic pyrophosphatase of Streptococcus faecalis. Interactions between polyamines and inorganic pyrophosphate.
We have shown a dual role for Mg2+ in the hydrolysis of PPi catalysed by inorganic pyrophosphatase (PPase; EC 3.6.1.1) of Streptococcus faecalis; Mg2+ is necessary for the formation of the substrates, Mg1PPi2- and Mg2PPi0, and it also acts as an allosteric activator [Lahti + Jokinen (1985) Biochemistry 24, 3526-3530]. No activity can be observed with S. faecalis PPase in the absence of bivalent...
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When the thiol-oxidizing agent diamide was added to a batch culture of Streptococcus faecalis during exponential growth, the specific activity of inorganic pyrophosphatase (EC 3.6.1.1) decreased to the level observed in the stationary phase. The effect of diamide was completely reversed by reduced glutathione. Furthermore, the ratio of reduced to oxidized glutathione and the specific activity o...
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چکیده ندارد.
15 صفحه اولTemperature-hydrostatic Pressure Studies on Partially Purified Inorganic Pyrophosphatase Activity.
Morita, Richard Y. (Oregon State University, Corvallis), and Paul F. Mathemeier. Temperature-hydrostatic pressure studies on partially purified inorganic pyrophosphatase activity. J. Bacteriol. 88:1667-1671. 1964.-Partially purified inorganic pyrophosphatase from Bacillus stearothermophilus demonstrated increased activity at 90 C with hydrostatic pressures of 100 to 1,100 atm. At 90 C, optimal ...
متن کاملLiver Microsomal Glucose 6-phosphatase, Inorganic Pyrophosphatase, and Pyrophosphate-glucose Phosphotransferase. Ii. Kinetic Studies.
Recently, we (1) described experiments which strongly supported the common identity of glucose 6-phosphatase,’ inorganic pyrophosphatase, pyrophosphate-glucose phosphotransferase, and mannose B-phosphate-glucose phosphotransferase activities present in a preparation obtained by ammonium sulfate fractionation of deoxycholate-dispersed rat liver microsomes. Previously, Segal, Washko, and Lee (2, ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1985
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2310485